Kar9 Asymmetrical Loading on Spindle Poles Mediates Proper Spindle Alignment in Budding Yeast
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چکیده
منابع مشابه
Asymmetric Loading of Kar9 onto Spindle Poles and Microtubules Ensures Proper Spindle Alignment
Spindle alignment is the process in which the two spindle poles are directed toward preselected and opposite cell ends. In budding yeast, the APC-related molecule Kar9 is required for proper alignment of the spindle with the mother-bud axis. We find that Kar9 localizes to the prospective daughter cell spindle pole. Kar9 is transferred from the pole to cytoplasmic microtubules, which are then gu...
متن کاملAsymmetric recruitment of dynein to spindle poles and microtubules promotes proper spindle orientation in yeast.
The orientation of the mitotic spindle plays a key role in determining whether a polarized cell will divide symmetrically or asymmetrically. In most cell types, cytoplasmic dynein plays a critical role in spindle orientation. However, how dynein directs opposite spindle poles toward distinct and predetermined cell ends is poorly understood. Here, we show that dynein distributes preferentially t...
متن کاملAssociation of mitochondria with spindle poles facilitates spindle alignment
Statistics of mitochondrial association with spindle poles in wild type and mto1∆ (Figures 1D and 2B) Out of the 35 imaged wild-type cells, 18 cells showed constant mitochondrial association (red bar in Figure 1D), 6 showed at least one spindle pole free from mitochondria for at least 5 minutes (green bar in Figure 1D), 6 displayed temporary mitochondrial detachment (not included in the compari...
متن کاملThe role of the proteins Kar9 and Myo2 in orienting the mitotic spindle of budding yeast
BACKGROUND Two genetic 'pathways' contribute to the fidelity of nuclear segregation during the process of budding in the yeast Saccharomyces cerevisiae. An early pathway, involving Kar9p and other proteins, orients the mitotic spindle along the mother-bud axis. Upon the onset of anaphase, cytoplasmic dynein provides the motive force for nuclear movement into the bud. Loss of either pathway resu...
متن کاملFocusing on spindle poles.
Spindle poles are discernible by light microscopy as the sites where microtubules converge at the ends of both mitotic and meiotic spindles. In most cell types centrosomes are present at spindle poles due to their dominant role in microtubule nucleation. However, in some specialized cell types microtubules converge into spindle poles in the absence of centrosomes. Thus, spindle poles in centros...
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ژورنال
عنوان ژورنال: Developmental Cell
سال: 2003
ISSN: 1534-5807
DOI: 10.1016/s1534-5807(03)00065-0